molecular evaluation of a case of visceral leishmaniasis due to leishmania tropica in southwestern iran
نویسندگان
چکیده
we describe a case of visceral leishmaniasis (vl) due to leishmania tropica in a 50-year-old iranian man lived in a vl-endemic area in southwest of iran. the patient presented with a 3-month history of fever and splenomegaly. clinical signs and serological findings were suggestive of vl. spleen biopsy was taken from the patient and intracellular forms of leishmania amastigotes was seen in giemsa stained smears. the patient was treated with pentavalent antimonial compound with complete resolution of his systemic signs and symptoms. dna was extracted from the microscopic slides of the spleen biopsy and the nagt (n-acetylglucosamine-1-phosphate transferase) gene of leishmania was pcr-amplified. sequence analysis of the pcr product demonstrated that the case has 99% identity with those of available sequences of l. tropica. intra-species variation within isolate was 0-0.1%; whereas, inter-species differences of the isolate with those of l. major and l. infantum was significantly higher.
منابع مشابه
Molecular Evaluation of a Case of Visceral Leishmaniasis Due to Leishmania tropica in Southwestern Iran
We describe a case of visceral leishmaniasis (VL) due to Leishmania tropica in a 50-year-old Iranian man lived in a VL-endemic area in southwest of Iran. The patient presented with a 3-month history of fever and splenomegaly. Clinical signs and serological findings were suggestive of VL. Spleen biopsy was taken from the patient and intracellular forms of Leishmania amastigotes was seen in Giems...
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We report the characterization of 6 Leishmania tropica isolates from 2 patients with visceral leishmaniasis who were unresponsive to treatment with sodium stibogluconate. The Leishmania isolates, MHOM/KE/81/NLB-029A, -029XIB, and -029XIC and MHOM/KE/81/NLB-030I, -030B, and -030XXA, all from splenic aspirates, were characterized by cellulose acetate electrophoresis using 11 enzymes: malate dehyd...
متن کاملEmergence of a new focus of visceral leishmaniasis due to Leishmania infantum in Golestan Province, north-eastern of Iran.
Over the last decade, a few cases of visceral leishmaniasis (VL) have been reported in some districts of the province of Golestan, in north-eastern Iran. The aim of the present study was to investigate the prevalence of Leishmania infantum infection among humans and domestic dogs by using direct agglutination test (DAT) and PCR assays in the eastern zone of the province. Between 2011 and 2012, ...
متن کاملleishmania tropica: another etiological agent of canine visceral leishmaniasis in iran.
background: dogs have been previously reported to be reservoirs of leishmania infantum as the etiological agent of human visceral leishmaniasis in iran. we report a case of canine visceral leishmaniasis (vl) caused by l. tropica from the north- west of iran where human visceral leishmaniasis is endemic. methods: the canine vl was initially screened by dipstick rk39 and direct agglutination test...
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عنوان ژورنال:
iranian journal of parasitologyجلد ۱۱، شماره ۱، صفحات ۱۲۶-۱۳۰
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